In 2004 he founded the NoSoftwarePatents campaign and in 2007 he provided some consultancy in relation to football policy. elena… Prof. Dr. Jaime Antonio Portilla Salinas Minsk, Weißrussland. 78 likes. Join ResearchGate to contact this researcher and connect with your scientific community. Cells expressing the 4G HIV-1 reporter and recorded for 8 hours at a rate of one 3D image stack every three minutes. In my Ph.D. During HIV-1 cytoplasmic journey, the viral core acts like a shell to protect the viral genetic material from antiviral sensors and ensure an adequate environment... Caenorhabditis elegans early embryos generate cell-specific transcriptomes despite lacking active transcription. For my postdoc I focused on gene expression, more specifically transcription, measured by imaging approaches (mRNA FISH and MS2). Only verified researchers can join ResearchGate and send messages to other members. Local translation allows for a spatial control of gene expression. Florian Müller (born January 21, 1970 in Augsburg, Germany) is an app developer[1] and an intellectual property activist. In this study, we used an improved SunTag system to label nascent proteins and image translation of single messenger ribonucleoproteins (mRNPs) in human cells. Neben zwei markanten Neubauten, dem Erweiterungsbau Ost und dem Neubau der Klinik für Geburtsmedizin werden zwei moderne Magistralen den gesamten Komplex auf der Nord- und Südseite neu erschließen. Geburtsort: Landshut Nationalität: Deutschland Position: Verteidiger Größe: 1,81 m Gewicht: 82 kg Schusshand: links bei ETC: 2012-2013 In diesem repräsentativen Ärztehaus mit 4 netten, sympathischen Ärztinnen www.spalenpraxis.ch, kann man sich nur wohlfühlen. Florian Müller (born January 21, 1970 in Augsburg, Germany) is an app developer and an intellectual property activist. Sehen Sie sich das Profil von Florian Müller im größten Business-Netzwerk der Welt an. Supplementary Figures 1-7, Supplementary Tables 1-2, Supplementary Notes 1-6 and Supplementary References. Transcription factor networks, together with histone modifications and signalling pathways, underlie the establishment and maintenance of gene regulatory architectures associated with the molecular identity of each cell type. Jetzt kostenlos registrieren & bis zu 10 CE Credits erhalten. Exhibition Manager – Halle 10. Cells dedicate significant energy to build proteins often organized in multiprotein assemblies with tightly regulated stoichiometries. The left panel shows a high contrast version of the right panel, to display single RNA molecules. Über uns; Veranstaltungen; Gruppenangebote; Kontakt © 2008-2021 ResearchGate GmbH. While many factors regulating cellular proliferation during liver repair have been identified, the mechanisms by which the injured liver maintains vital functions prior to tissue recovery are unknown. Imaging techniques allow their observation at high spatial and temporal resolution, while keeping cellular morphol... Lymphocytic choriomeningitis mammarenavirus (LCMV) is an enveloped, negative-strand RNA virus that causes serious disease in humans but establishes an asymptomatic, lifelong infection in reservoir rodents. Florian Müller. [23] The lawsuit ended with both parties agreeing to zero dollars in statutory damages for a small amount of copied code, so that Oracle could appeal. To address this uncertainty, we compare fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a transcription factor, the glucocorticoid receptor, in the nuclei of... We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. [25] In a court filing in the Oracle v. Google case, Oracle stated that it paid Florian Müller as a consultant. [21] ArsTechnica's Ryan Paul also said that these findings in the online codebase are also not evidence that copyright infringing code is distributed on Android handsets. 24,891 likes. The in vivo binding interactions of several different transcription factors with chromatin have been investigated recently using quantitative fluorescence recovery after photobleaching (FRAP). Competition ChIP relies on a cell containing two copies of a single DNA-associated factor, with each copy of the factor differentially epitope tagged. Transcription is a stochastic process occurring mostly in episodic bursts. In order to replicate, human immunodeficiency virus (HIV-1) reverse-transcribes its RNA genome into DNA, which subsequently integrates into host cell chromosomes. Here, we investigated the recruitment of Rev to the transcription sites of HIV-1 reporters that splice either post- or cotranscriptionally. Physiotherapie Basel Santewell - Ihr Kompetenzzentrum für Physiotherapie mit erfahrenen Physiotherapeuten! These two sta... Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. FRAP can be applied qualitatively to assess if chromatin binding interactions are altered by various biological perturbations. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Cells contain many subcellular structures in which specialized proteins locally cluster. Here, we investigated this localization mechanism. According to Ed Burnette, a ZDNet blogger, Google published those files on its web site to help developers debug and test their own code. He consulted for Microsoft and writes the FOSSPatents blog about patent and copyright issues. Sorry, you need to be a researcher to join ResearchGate. PRC1 Components Exhibit Different Binding Kinetics in Polycomb Bodies. See a recent post on Tumblr from @salzigersee about mainz05. Sei Teil des größten Online-Events von Amann Girrbach! Elena A. Algaer, Florian Müller-Plathe. Im Profil von Florian Kremser sind 3 Jobs angegeben. Die offizielle Seite des Schauspielers Florian Stadler In mammals, a class of mRNAs localize to cytoplasmic protrusions in an APC-dependent manner, with roles during cell migration. E-Mail senden. Institut de Biologie de l’Ecole Normale Supérieure (IBENS), French Institute of Health and Medical Research, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Institut de Génétique Moléculaire de Montpellier, Department of Developmental Biology and Stem Cell Development, Spatial transcriptomics for respiratory research and medicine, A choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport, Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH, Clustering and reverse transcription of HIV‐1 genomes in nuclear niches of macrophages, The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions, Functional compensation precedes recovery of tissue mass following acute liver injury, A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting, mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage, A localization screen reveals translation factories and widespread co-translational RNA targeting, Clustering and reverse transcription of HIV-1 genomes in nuclear niches of macrophages, Remodeling of the Core Leads HIV-1 Preintegration Complex into the Nucleus of Human Lymphocytes, Functional Compensation Precedes Recovery of Tissue Mass Following Acute Liver Injury, Remodeling of the core leads HIV-1 pre-integration complex in the nucleus of human lymphocytes, ImJoy: an open-source computational platform for the deep learning era, Co-translational assembly of mammalian nuclear multisubunit complexes, A Deep Learning Approach To Identify MRNA Localization Patterns, The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells, A computational framework to study sub-cellular RNA localization, Co-translation drives the assembly of mammalian nuclear multisubunit complexes, A Growing Toolbox to Image Gene Expression in Single Cells: Sensitive Approaches for Demanding Challenges, The molecular logic of Nanog-induced self-renewal, Visualization of arenavirus RNA species in individual cells by single-molecule fluorescence in situ hybridization (smFISH) suggests a model of cyclical infection and clearance during persistence, A Localization Screen Reveals Translation Factories and Widespread Co-Translational Protein Targeting, Mitotic binding of Esrrb marks key regulatory regions of the pluripotency network, Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells, SmiFISH and FISH-quant - A flexible single RNA detection approach with super-resolution capability, Single molecule tracking of Ace1p in Saccharomyces cerevisiae defines a characteristic residence time for non-specific interactions of transcription factors with chromatin, A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting, Divergent Binding and Transactivation by Two Related Steroid Receptors at the Same Response Element, Distinctly Different Dynamics and Kinetics of Two Steroid Receptors at the Same Response Elements in Living Cells, Transcription Factors Modulate c-Fos Transcriptional Bursts. The linker histone H1 has a fundamental role in DNA compaction. These two key events of the viral life cycle are commonly viewed as separate not only in time but also in cellular space, since reverse transcription (RT) is thought to be completed i... Retroviral replication proceeds through obligate integration of the viral DNA into the host genome. He initiated and managed such alliances in various market segments, including productivity software, utility software, educational software, and computer games. This presents an opportunity to study mechanisms of post-transcriptional regulatory control. Single molecule FISH (smFISH) allows studying transcription and RNA localization by imaging individual mRNAs in single cells. [24], In April 2012, Müller said he had been hired by Oracle to consult on competition-related topics including FRAND licensing terms. Willkommen zur AG. Translation is an essential step in gene expression. From 2001 to 2004, Müller advised the CEO of MySQL AB,[7] developer of the namesake open-source database management software product. They accumulate into nuclear foci called Polycomb bodies, which function in Drosophila cells as silencing compartments where PcG target genes convene. IT-focused website Silicon.com listed him among the Silicon Agenda Setters. in living cells, and chromatin immunoprecipitation (ChIP) analysis, measured in minutes. Total time is 15 min, frame rate is one 3D stacks per 3 seconds. Auf LinkedIn können Sie sich das vollständige Profil ansehen und mehr über die Kontakte von Florian Müller und Jobs bei ähnlichen Unternehmen erfahren. Kultur und Kunst un der Bernstrasse Atelierplätze und Kunst- Veranstallungsraum als Zwischennutzung 33 were here. Wer sich der Praxis hingibt ohne Wissenschaft, ist wie der Steuermann, der ein Schiff ohne Ruder und Kompass besteigt und nie weiß, wohin er fährt. Menschen lernen Jesus kennen. In my current position I will develop methods to study diverse biological questions with a special focus on mRNA biogenesis and nuclear organization. These two key events of the viral life cycle are commonly viewed as separate not only in time, but also in cellular space, since reverse transcription (RT) is thought to be completed in t... RNA localization and local translation are important for numerous cellular functions. Berufserfahrung, Kontaktdaten, Portfolio und weitere Infos: Erfahr mehr – oder kontaktier Nick Breit direkt bei XING. [4] A year later, at age 16, he became Germany's youngest computer book author. [27], Müller amplified[28] a Huffington Post article by Edward Naughton, an intellectual property lawyer[29] who has previously represented Microsoft,[30][31] who suggested that Google likely violated the GPL by copying Linux header files. Dissecting the binding mechanism of the linker histone in live cells: An integrated FRAP analysis, Regulation of TATA-binding protein dynamics in living yeast cells, Intracellular delivery of acetyl-histone peptides inhibits native bromodomain–chromatin interactions and impairs mitotic progression, Evidence for a Common Mode of Transcription Factor Interaction with Chromatin as Revealed by Improved Quantitative Fluorescence Recovery after Photobleaching, Analysis of Binding at a Single Spatially Localized Cluster of Binding Sites by Fluorescence Recovery after Photobleaching, Department of Biology and Stem Cell Development, Department of Cell Biology and Infection of Paris. [5], From 1987 to 1998, he specialized on publishing and distribution cooperations between US and European software companies. FRAP and kinetic modeling in the analysis of nuclear protein dynamics: What do we really know? Cells dedicate significant energy to build proteins often organized in multiprotein assemblies with tightly regulated stoichiometries. Florian Mueller Florian Mueller is an app developer who used to be an award-winning intellectual property activist. The left panel shows a high contrast version of the right panel, to display single RNA molecules. Telefon +49 711 61946-455. His 30 years of software industry expertise span different market segments (games, education, productivity and infrastructure software), diverse business … Stable assembly of HIV-1 export complexes occurs cotranscriptionally. He consulted for Microsoft and writes the FOSSPatents blog about patent and copyright issues. Florian Schmitt Informationsvideos Kontakt Herzlich Willkommen bei der Steuerberatung Schmitt. Convoys arise by a Mediator-dependent reinitiation mechanism, which generates a tra... An isolated transcription cycle in a HeLa cell expressing the WT HIV-1 reporter. During the HIV-1 cytoplasmic journey, the viral core acts as a shell to protect the viral genetic material from antiviral sensors and ens... Retroviral replication proceeds through obligate integration of the viral DNA into the host genome. Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. [2][3] From 1985 to 1998, he was a computer magazine writer and consultant for companies, helping with collaborations between software companies. The spatiotemporal regulation of gene expression is key to many biological processes. In order to replicate, the Human Immunodeficiency Virus (HIV-1) reverse transcribes its RNA genome into DNA, which subsequently integrates into host cell chromosomes. As a consultant to and representative of Blizzard Entertainment, Müller was involved in their marketing campaigns. Florian Stadler. Conventional chromatin immunoprecipitation (ChIP) experiments measure transcription factor occupancy, but give no indication of kinetics... Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring the mobility of fluorescently tagged proteins in living cells. Seit sich Michael Wendler als Corona-Skeptiker geoutet hat, lebt er zurückgezogen mit Frau Laura in Florida. National Academy of Science of Belarus. Although FRAP presumes that high-intensity illumination causes only irreversible photobleaching, reversible photoswitching of many fluorescent molecules, including GFP, can also occur. Cells expressing the WT HIV-1 reporter and recorded for 8 hours at a rate of one 3D image stack every three minutes. To address this issu... Live-cell imaging has revealed unexpected features of gene expression. In 1996, he co-founded an online gaming service named Rival Network,[6] which in early 2000 was acquired by the Telefónica group. Termine sofort verfügbar! Evangelisch-Freikirchliche Gemeinde Wölmersen. Although pathways for assembly of RNA polymerase (Pol) II transcription preinitiation complexes (PICs) have been well established in vitro, relatively little is known about the dynamic behavior of Pol II general transcription factors in vivo. Here, we performed a dual protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. Exhibition Manager – Halle 3. The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Binding can now be quantified in live cells, but the accuracy of such measurements remains uncertain. Das Bauvorhaben am Klinikum Oldenburg gliedert sich in vier konkrete Maßnahmen. From left to right: Ricardo Henriques, Hua Wong, Claas von Middendorff, Christophe Zimmer, Florian Müller, Mickaël Lelek, Hervé Marie-Nelly, Marie-Anne Lin RECENT ALUMNI Elena RENSEN, postdoc An dieser Stelle werden die Namen der ausgelosten Stipendiat*innen bekannt gegeben. The cerebellum influences sensorimotor adaptation by modulating sensorimotor plasticity of the primary motor cortex. Berufserfahrung, Kontaktdaten, Portfolio und weitere Infos: Erfahr mehr – oder kontaktier Elena Stengler direkt bei XING. Using simulated images, we design and validate a set of features describing different RNA localization patterns including polarize... A bstract Montag Dienstag Mittwoch Donnerstag Freitag 7.30 - 19.00 Uhr 7.30 - 17.00 Uhr Ao. Her... Fluorescence recovery after photobleaching (FRAP) has now become widely used to investigate nuclear protein binding to chromatin in live cells. In 1985, Müller started writing articles for German computer magazines. [8] Following several years of intensive lobbying by many parties, this proposed directive was rejected by the European Parliament on July 6, 2005, with 648 out of 680 votes cast. The positive-sense single-stranded RNA virus contains a single linear RNA segment that serves as a template for transcription and replication, leading to the synthesis of positive and negative-stranded viral RNA (vRNA) in infected cells. The binding of nuclear proteins to chromatin in live cells has been analyzed by kinetic modeling procedures applied to experimental data from fluorescence recovery after photobleaching (FRAP). Sehen Sie sich das Profil von Florian Müller im größten Business-Netzwerk der Welt an. Recent imaging approaches opened exciting perspectives for understanding the intricate mechanisms regulating RNA metabolism, from synthesis to decay. Profile von Personen mit dem Namen Florian Mueller anzeigen. A leading publication for intellectual property lawyers, "Managing Intellectual Property", counted Müller – along with the Chinese vice premier Wu Yi – among the "top 50 most influential people in intellectual property"[11][12] (renominated in 2006[13]). Seit dem ersten Schritt in diese Liegenschaft habe ich mich in diese einmalige Atmosphäre verliebt!. Binding interactions within such clusters may be analyzed in live cells using models for fluorescence recovery after photobleaching (FRAP). Although models for H1 binding generally involve the H1 C-terminal tail and sites S1 and S2 within the H1 globular domain, there is debate about the importance of these binding regions and almost nothing is known about how they work together. Die Namen werden in alphabetischer Reihenfolge (Nachname, Vorname) dargestellt. In vitro, a subset of Pol II factors facilitates reinitiation by remaining very stably bound to the promote... Bromodomains present in Brd4 and other chromatin proteins interact with acetylated histones to regulate transcription and cell growth. [34], proposed directive on the patentability of computer-implemented inventions, "Microsoft asks ITC to compel Google to provide info in BN case", "Torvalds comes out against EU patent directive", European Parliament rejects computer‐implemented inventions directive, "Revealed: 2005's most influential people", "Anti-patent campaigner hailed as IP hero", "Comercialización del fútbol europeo: el otro alegato", "FOSS Patents: New evidence supports Oracle's case against Google", "Oops: No copied Java code or weapons of mass destruction found in Android", "New alleged evidence of Android infringement isn't a smoking gun", "Android device makers distribute Oracle code online (Motorola, LG, Samsung)", "Oracle agrees to 'zero' damages in Google lawsuit, eyes appeal", "Oracle v. Google trial: evidence of willful infringement outweighs claims of approved use", "Oracle names bloggers, others it paid to comment on Google trial", "Oracle confirms paying a blogger but Google names no-one", "Google's Android faces a serious Linux copyright issue (potentially bigger than its Java problem)", "Google's Android Contains Legal Landmines for Developers and Device Manufacturers", "Open Source Report: Lawyer behind Android infringement claim has Microsoft ties", "Android: Sued by Microsoft, not by Linux", "Oracle and Google File Paid Writers Lists", "Microsoft legal win over Google may signal ceasefire | Mobile - CNET News", https://en.wikipedia.org/w/index.php?title=Florian_Müller_(author)&oldid=987181074, Articles with unsourced statements from January 2017, Wikipedia articles with WORLDCATID identifiers, Creative Commons Attribution-ShareAlike License, This page was last edited on 5 November 2020, at 12:27. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. [9][10], For his political activities, Müller received several awards in 2005. Florian Mueller RNA localization is a crucial process for cellular function and can be quantitatively studied by single molecule FISH (smFISH). Sehen Sie sich das Profil von Florian Kremser im größten Business-Netzwerk der Welt an. In particular, for the HIV-1 genome to enter the nucleus, it must be led through the nuclear pore complex (NPC). It possesses unique regenerative capacity upon injury. Innrain 52 d, 6020 Innsbruck The left panel shows a high contrast version of the right panel, to display single RNA molecules. Soft Materials 2012 , 10 (1-3) , 42-80. PcG proteins also exert their activities elsewhere in the nucleoplasm. E-Mail senden. 32 mRNAs displayed specific cytoplasmic localizations with local translation at unexpected locations, including cytoplasmic protrusions, cell edges, endosomes, Gol... Caenorhabditis elegans early embryos generate cell-specific transcriptomes despite lacking active transcription, thereby presenting an opportunity to study mechanisms of post-transcriptional regulatory control. Fulda (oz/jb) – Die Abgänger und Abgängerinnen der Richard-Müller-Schule in Fulda. Motor cortex sensorimotor plasticity is maladapt... Competition chromatin immunoprecipitation (competition ChIP) enables experimenters to measure protein-DNA dynamics at a single locus or across the entire genome, depending on the detection method. From 1985 to 1998, he was a computer magazine writer and consultant for companies, helping with collaborations between software companies. However, how master transcription factors individually impact the epigenomic landscape and orchestrate the behaviour of regu... RNA localization is a crucial process for cellular function and can be quantitatively studied by single molecule FISH (smFISH). Here, we performed a dual protein/mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. Here, we use high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. Die hohen Decken, die Mischung aus Alt/Neu, den Stuckaturen an der Decke und der imposanten Innenausstattung, dem alten Eichenboden, sind nur einzelne Highlights.. Total time is 15 min, frame rate is one 3D stacks per 3 seconds. I'm a trained biophysicist and develop integrated approaches combing imaging, computerized image analysis, and mathematical modeling. Im Profil von Florian Müller sind 4 Jobs angegeben. Due to our privacy policy, only current members can send messages to people on ResearchGate. Im Profil von Florian Müller sind 4 Jobs angegeben. Leonardo da Vinci. In vivo single molecule tracking has recently developed into a powerful technique for measuring and understanding the transient interactions of transcription factors (TF) with their chromatin response elements. I studied the mobility of different nuclear proteins in living cells. Deep learning methods have shown extraordinary potential for analyzing very diverse biomedical data, but their dissemination beyond developers is hindered by important computational hurdles.
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